The Leica SL laser scanning confocal microscope
The Leica SL laser scanning confocal system consists of an upright microscope with two detector channels and three lasers generating six lines for fluorescence excitation (458, 476, 488, 514, 543, 633 nm). Emissions are split using fixed dichroic mirrors. The system contains spectral scanning and three-dimensional acquisition/display capabilities. Emissions are split into a spectrum using a prism and are collected through two separate tunable emission slits/photomultiplier tubes. A parallel transmitted light image can be collected. Currently available objectives on the system are: 63x oil immersion (Plan-Apo, 1.4 NA), 40x oil immersion (Plan-Apo, 1.25 NA), and 10x multi-immersion (oil, water, glycerin) (Plan-Apo, 0.4 NA). The SL microscope is located in HSW-1142.